CAPILLARYS HEMOGLOBIN(E)

Hemoglobin analysis by capillary electrophoresis: a clear pattern for easy interpretation

TEST PRESENTATION

Hemoglobin electrophoresis is a well-established technique routinely used in clinical laboratories to screen samples for hemoglobinopathies (hemoglobin variants and thalassemias).

The CAPILLARYS HEMOGLOBIN(E) kit enables, on CAPILLARYS instruments, the efficient separation of hemoglobin fractions and detection of a large number of hemoglobin variants and thalassemias patterns.

CAPILLARYS instruments perform all sequences automatically, from sampling to final clear-cut profile, with precise quantification and presumptive identification of the most common hemoglobins.

CAPILLARYS instruments allow high resolution separation of the major hemoglobin variants (Hb S, Hb C, Hb D and Hb E) and accurate quantification of the Hb A2 and Hb F.

Hb H and Bart’s, as seen in alpha-thalassemia, are clearly separated and easily quantified.

The CAPILLARYS HEMOGLOBIN(E) kit allows hemoglobin analysis on adult and newborn cord blood samples.

 

TEST PRINCIPLE

The CAPILLARYS HEMOGLOBIN(E) assay is based on the principle of capillary electrophoresis in free solution. Hemoglobin fractions are separated in silica capillaries, by their electrophoretic mobility and electroosmotic flow at a high voltage in an alkaline buffer. Hemoglobin fractions are directly detected at the specific absorbance of 415 nm. The pattern is divided into 15 zones (12 zones for cord blood). Each zone displays a drop down library of possible variants migrating within this zone. More than 300 variants are listed.

CAPILLARYS HEMOGLOBIN(E) users can request from their Sebia representative the HEMOGLOBINS ATLAS CD for more information.

 

performances

  • Throughput: 36 tests/h
  • Excellent precision and linearity on Hb A, Hb F, Hb A2 and common variants measurements
  • Full traceability and positive ID for samples and reagents
  • CAPILLARYS 2 FLEX PIERCING: cap piercing capacity and whole blood primary tube mixing

kit description

CAPILLARYS HEMOGLOBIN(E) (PN 2007) kit content:

  • Buffer
  • Hemolyzing solution
  • Wash solution
  • Dilution segments
  • Filters

Related products and accessories not provided in the kit:

  • Quality controls:
    • Normal Hb A2 control (PN 4778)
    • Pathological Hb A2 control (PN 4779)
    • Hb AF control (PN 4777)
    • Hb AFSC control (PN 4792)
  • Tubes and caps for control (PN 9202, 9205)
  • Wedge adapter (PN 9203)
  • Boxes for control storage (PN 2082)
  • CAPICLEAN (PN 2058)
  • HEMOGLOBINS ATLAS
  • CAPIprotect (2 x 5L) (PN 2061)

Compatible Instruments

NOTE: Contact your Sebia representative to verify the product availability in your country.