HYDRAGEL ACID(E) HEMOGLOBIN(E) 

Gel electrophoresis for second-line hemoglobin testing

Test presentation

Hemoglobin electrophoresis is a well-established technique routinely used in clinical laboratories to confirm samples for hemoglobinopathies (hemoglobin variants and thalassemias).

The HYDRAGEL ACID(E) HEMOGLOBIN(E) kit enables, on HYDRASYS instruments, the separation of the normal hemoglobins (A and F) and the detection of the major hemoglobin variants: S and C, by electrophoresis on acidic agarose gels.

The semi-automated HYDRASYS instrument offers sample application, electrophoretic migration and staining. 

The visualization of the gel provides qualitative analysis.

Electrophoresis on acidic gel is used to differentiate hemoglobins S from D and E from C.

 

Test principle

The HYDRAGEL ACID(E) HEMOGLOBIN(E) assay is based on the principle of electrophoresis separation on agarose gel at acidic pH (pH 5.9).

Prior to performing the test, it is mandatory to prepare the samples (hemolyzed washed red blood cells).

The HYDRASYS system is a semi-automated multi-parameter instrument. The automated steps include processing of the agarose gel in the following sequence:

  • Sample application
  • Electrophoretic migration
  • Drying
  • Staining with amidoblack dye
  • Destaining
  • Final drying 

Performances

  • Resolution:
    • Optimal focalization of the fractions obtained by applying a very thin sample’s application using the Sebia proprietary applicators.

Kit description

HYDRAGEL ACID(E) HEMOGLOBIN(E) kit: 10 gels of 7 or 15 tests

  • HYDRAGEL 7 ACID(E) HEMOGLOBIN(E) (PN 4108)
  • HYDRAGEL 15 ACID(E) HEMOGLOBIN(E) (PN 4128)

Kit content:

  • Agarose gels
  • Buffered strips
  • Ethylene glycol solution
  • Hemolyzing solution
  • Amidoblack stain
  • Staining solution diluent
  • Applicators
  • Filter papers

Related product and accessories not included in the kit:

  • Quality controls:
    • Normal Hb A2 Control (PN 4778)
    • Pathological Hb A2 Control (PN 4779)
    • Hb AFSC Control (PN 4792)
  • Destaining Solution (PN 4540)
  • HYDRASYS Wash Solution (PN 4541)

Compatible Instruments

HYDRASYS

HYDRASYS FOCUSING

HYDRASYS 2

HYDRASYS 2 SCAN

HYDRASYS 2 FOCUSING

HYDRASYS 2 SCAN FOCUSING

GELSCAN

NOTE: Contact your Sebia representative to verify the product availability in your country.