HYDRAGEL ISO-PAL

Full separation of all phosphatase alkaline’s isoforms in one gel

Test presentation

Electrophoresis of the Alkaline Phosphatase isoenzymes is a well-established technique routinely used in clinical laboratories for screening samples for various diseases (e.g. bone and liver metastasis, ovarian tumors, etc).

On the HYDRASYS instruments, utilizing the HYDRAGEL ISO-PAL gels, serum alkaline phosphatase isoenzymes are separated (bone, liver, placental and intestinal fractions).

The semi-automated HYDRASYS instrument offers sample application, electrophoretic migration and detection. 

The visualization of the gel provides qualitative analysis, while the reading of the agarose gels on a Sebia reader provides alkaline phosphatase profiles for relative quantitative analysis.

On the HYDRASYS 2 SCAN, the scanning system is embedded in the instrument.

 

Test principle

The HYDRAGEL ISO-PAL assay is based on the principle of lectin affinity, in agarose gel electrophoresis. To perform the test, each sample is applied in duplicate. In the course of migration, one of the sample duplicate passes through lectin deposited anodally from the sample’s point of application (the lectin itself shifts only slightly cathodally).

The HYDRASYS system is a semi-automated multi-parameter instrument. The automated steps include processing of the agarose gel in the following sequence:

  • Sample and lectin application
  • Electrophoretic migration
  • Incubation with substrate
  • Blotting
  • Washing
  • Final drying 

Performances

  • Sensitivity:
    • The lowest detected activity of the individual isoenzymes by densitometry ranged from 1 to 4 IU/L.

Kit description

HYDRAGEL ISO-PAL kit: 10 gels of 3 or 7 tests

  • HYDRAGEL 7 ISO-PAL (PN 4112)
  • HYDRAGEL 15 ISO-PAL (PN 4132)

Kit content:

  • Agarose gels
  • Buffered strips
  • Lectin
  • Substrate
  • Chromogen
  • Applicators
  • Filter papers (thick and thin)
  • Plastic masks

Related product and accessories not included in the kit:

  • Quality control: ISO-PAL control (PN 4793)
  • Accessory kit for HYDRASYS ISO-LDH, ISO-PAL, CHOL (PN 1261)
  • Destaining solution (PN 4540)
  • HYDRASYS wash solution (PN 4541)

Compatible Instruments

HYDRASYS

HYDRASYS FOCUSING

HYDRASYS 2

HYDRASYS 2 SCAN

HYDRASYS 2 FOCUSING

HYDRASYS 2 SCAN FOCUSING

GELSCAN

NOTE: Contact your Sebia representative to verify the product availability in your country.