HYDRAGEL URINE PROFIL(E) 

The most complete “all-in-one” solution for proteinuria investigation by electrophoresis 

Test presentation

Protein immunofixation is a well-established technique for typing any suspected monoclonal component on urine electrophoresis. It also allows the characterization of the proteinuria.  

The HYDRAGEL URINE PROFIL(E) kit is used for proteinuria investigation by immunofixation on agarose gel in order to:

  • Detect and type Bence Jones proteins (monoclonal kappa and/or lambda free light chains) or any other monoclonal full immunoglobulin.
  • Type renal lesions (tubular, glomerular or mixed lesion).
  • Quantify the monoclonal peak.

 

The detection of any Bence Jones proteins or renal damage is essential for patients with multiple myeloma or other plasma cell dyscrasias.

The semi-automated HYDRASYS instrument performs the migration, the immunofixation and the staining of the agarose gels, in order to obtain protein profiles for qualitative analysis. The sensitivity of HYDRAGEL URINE PROFIL(E) permits the detection of very small monoclonal components.

A recent development on GELSCAN offers the quantification of the monoclonal protein on the ELP (Protein Electrophoresis) track.

Test principle

The HYDRAGEL URINE PROFIL(E) assay is based on the principle of agarose gel electrophoresis followed by immunofixation. After separation of urine proteins according to their charge, the gel is incubated with different specific antisera targeted against:

  • Low molecular weight serum proteins revealing tubular damage:
    • ß2 microglobulin,
    • Retinol Binding Protein (RBP)
    • α1 microglobulin
  • High molecular weight serum proteins revealing glomerular damage:
    • Heavy chains Gamma, Alpha & Mu (with a trivalent antiserum),
    • Heavy chains Delta & Epsilon (with a divalent antiserum) (SM*)
    • Albumin
  • α2 macroglobulin revealing blood contamination
  • Kappa (κ) and Lambda (λ) free and bound light chains
  • Kappa (κ) and Lambda (λ) free light chains.

The gel is then processed in order to remove unbound proteins before the final staining step. The gel is inspected visually and the monoclonal peak can be quantified by scanning the ELP track.

Performances

  • Sensitivity:
    • The minimal detection limit is about:
      • 6 mg/L for tubular proteins
      • 3 mg/L for glomerular proteins
      • 6 mg/L for Ig D
      • 12 mg/L for Ig E
    • The detection limit of the Bence Jones protein is about:
      • 20 mg/L with anti-free and bound light chains antisera
      • 50 mg/L with anti-free light chains antisera.
    • According to the mobility of the monoclonal component and polyclonal background, the sensitivity may vary.
  • Full traceability and positive ID are obtained by connecting the HYDRASYS to the ASSIST pipetting station

Kit description

HYDRAGEL URINE PROFIL(E) kit: 10 gels of 2 or 4 tests

  • HYDRAGEL 2, 4 URINE PROFIL(E) (SM*) (PN 4831, 4832)
  • HYDRAGEL 2, 4 URINE PROFIL(E) (DM**) (PN 4331, 4332)

Kit content:

  • Agarose gels
  • Buffered strips
  • Acid violet stain
  • Applicators
  • Filter papers (thick and thin)
  • Filter paper combs*
  • Antisera segments**

Related products and accessories not provided in the kit:

  • Quality control: IT/IF control (PN 4788)
  • Dynamic mask (PN 1255)
  • URINE PROFIL(E) Mask (SM*) (PN 1273)
  • (GAM, κ and λ) antisera and fixative solution kit (SM*) (PN 4835)
  • (GAM, κ and λ) antisera and fixative solution kit (DM**) (PN 4335)
  • Antisera κ and λ free light chains (SM*) (PN 4836)
  • Antisera κ and λ free light chains (DM**) (PN 4336)
  • Anti-Ig DE (SM*) (PN 4837)
  • Antisera kit for URINE PROFIL(E) (SM*) (PN 4838)
  • Antisera kit for URINE PROFIL(E) (DM**) (PN 4338)
  • Destaining solution (PN 4540)
  • HYDRASYS wash solution (PN 4541)
  • FLUIDIL (PN 4587)

Compatible Instruments

HYDRASYS

HYDRASYS FOCUSING

HYDRASYS 2

HYDRASYS 2 SCAN

HYDRASYS 2 FOCUSING

HYDRASYS 2 SCAN FOCUSING

ASSIST

GELSCAN

* Only with Sebia Standard Mask (SM)

** Only with Sebia Dynamic Mask (DM)

NOTE: Contact your Sebia representative to verify the product availability in your country.