CAPILLARYS CDT

A specific biomarker for chronic alcohol abuse by automated capillary electrophoresis at a high throughput

Test presentation

Quantification of Carbohydrate Deficient Transferrin (CDT) by electrophoresis is used for screening patient serum samples to detect chronic alcohol abuse. During analysis, serum transferrin isoforms are separated into five major fractions according to their sialylation level:

  • Asialotransferrin (non-sialylated)
  • Disialotransferrin
  • Trisialotransferrin
  • Tetrasialotransferrin
  • Pentasialotranferrin

CDT percent value is automatically calculated using the sum of low-sialylated isoforms, i.e., disialotransferrin associated with asialotransferrin (if present). CDT levels equal to or less than 1.3% are considered normal, but CDT levels over 1.6% are abnormal and indicative of chronic alcohol abuse. CDT values over 1.3% and below 1.6% are inconclusive results.  

The CAPILLARYS CDT kit allows automatic quantification of CDT with visualization of all transferrin isoforms. Curve abnormalities can thus be detected which prevent false result reporting (such as in cirrhotic pattern).

 

Test principle

The CAPILLARYS CDT assay is based on the principle of capillary electrophoresis in free solution. Transferrin isoforms are separated in silica capillaries by their electrophoretic mobility and electroosmotic flow at high voltage in an alkaline buffer. Transferrin isoforms are directly detected during migration by UV absorbance.

All analysis steps are performed automatically. The sample dilution is done using a specific diluent allowing iron saturation.

 

Performances

  • Throughput: 38 tests/h
  • Full traceability and positive ID for samples and reagents
  • Rapid validation through color-coded profiles using the mosaic screen

Kit description

CAPILLARYS CDT (PN 2009) kit content:

  • Buffer
  • Sample diluent
  • Wash solution
  • CDT wash solution
  • Dilution segment
  • CDT wash segment
  • Filters

Related products and accessories not provided in the kit:

  • Quality controls:
    • Normal CDT Control (PN 4795)
    • High CDT Control (PN 4772)
  • CAPICLEAN (PN 2058)
  • CAPILLARYS / MINICAP CDT samples treatment solution (PN 2054)
  • CDT / IS TETRAVALENT (PN 2057)
  • Blue dilution segments (PN 2083)
  • CAPIprotect (2 x 5L) (PN 2061)

compatible Instruments

CAPILLARYS 2

CAPILLARYS 2 FLEX-PIERCING

NOTE: Contact your Sebia representative to verify the product availability in your country. 

Other products associated