Alpha 1-Antitrypsin (A1AT) Phenotype Testing
The gold standard assay for the alpha 1-antitrypsin (A1AT) phenotype testing.
About A1AT
The most common method to detect A1AT deficiency is protein phenotyping using isoelectric focusing.
The HYDRAGEL 18 A1AT ISOFOCUSING kit used on the HYDRASYS 2 SCAN FOCUSING instrument provides qualitative detection of the different phenotypes of the A1AT in human serum.
The semi-automated HYDRASYS 2 instrument enables migration, immunofixation and direct detection on agarose gels, to obtain A1AT profiles for qualitative analysis.
Phenotyping results in conjunction with clinical findings and other laboratory assays aid in the diagnosis of Alpha 1-antitrypsin deficiency.
Key benefits
Accurate Diagnosis
100% of concordance with another agarose gel isocelectric focusing and in agreement with the different phenotypes.
Efficient Method
Clear band visualization for a reliable interpretation.
Fast result
Simple step-by-step procedure without membrane transfer for 2.5 hours of turnaround time.
Testing is part of algorithms
HYDRAGEL 18 A1AT ISOFOCUSING is included in both screening and diagnostic algorithms to provide clinicians with phenotype of A1AT proteins secreted in blood circulation.
Main characteristics
The HYDRAGEL 18 A1AT ISOFOCUSING assay is based on the principle of agarose gel isoelectrofocusing followed by immunofixation.
- After the protein separation according to their isoelectric point, the gel is directly incubated with a peroxidase labelled anti-A1AT antiserum with no need for a membrane blotting step.
- Then, the gel is processed in order to remove unbound proteins before the enzymatic reaction.
- Finally, the gel is visually evaluated.
Human serum samples can be used with HYDRAGEL 18 A1AT ISOFOCUSING.
- 2 stages-protocol: Isoelectrofocalisation and Immunofixation for an optimized result
- A1AT controls (MM, MZ and MS) are included in each series of analysis to quickly identify M, S and Z phenotypes.
10 gels of 18 tests.
- HYDRAGEL 18 A1AT ISOFOCUSING – P/N: 4357
Featured instruments for A1AT
See all instruments
Related resources
See all resourcesApr. 11, 2022 Nov. 21, 2024
IFCC Euromedlab 2021
Sebia booth: #121
Sebia Workshop: EduW22
Jun. 27, 2022 Jun. 29, 2022
IFCC Worldlab 2022
Sebia booth: #72
Sebia Workshop: EduW25
Improving the diagnosis of AATD with aid of Serum Protein Electrophoresis
Educational Webinar with Dr. Ilaria Ferrarotti
Scientific publications
See all publicationsPitfalls and ceveats of A1AT diagnosis
Pitfalls and caveats in α1-antitrypsin deficiency testing: a guide for clinicians.
Evaluation of Sebia’s isoelectrofocusing kit for A1AT
Evaluation of Sebia’s isoelectrofocusing kit for A1AT with HYDRASYS.
Interested in A1AT?
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Please contact your local Sebia representative. Information intended for healthcare professionals.
Carefully read the instructions in the reagent package inserts and instrument manuals.