von Willebrand
Multimer Analysis
Multimer assay simplified.
About von Willebrand Multimer Analysis
von Willebrand disease (vWD) is a genetic bleeding disorder caused by missing or defective von Willebrand factor (vWF).
Since von Willebrand disease (vWD) is a complex disease, its diagnosis requires a large panel of tests for an accurate diagnosis and classification of vWD. Multimer analysis is an integral part of the diagnostic process and an essential information for the clinicians for an optimal patient management.
HYDRAGEL von WILLEBRAND MULTIMERS kit is designed for the detection of abnormalities in the vWF multimers distribution related to a haemorrhagic diathesis. The test allows to assess the overall size distribution of von Willebrand factor multimers in human plasma using agarose gel electrophoresis and immunofixation, with the semi-automated HYDRASYS 2 instrument.
Key benefits
Semi-automated test
providing standardised and reproducible results.
Simplified technique
making this test accessible to any laboratory.
Within-day results
compared to in house tests delivering results in 4-5 days.
Traceability of the result
with densitometry-based interpretations.
Main characteristics
HYDRAGEL von WILLEBRAND MULTIMERS allows separating small, intermediate, and large molecular weight multimers.
The assay is carried out in two stages:
- Sample pre-treatment followed by an electrophoresis done in SDS agarose gel to separate proteins according to their molecular weight,
- direct immunofixation of multimers in the gel using anti-human vWF antibody followed by immunoenzyme staining to visualize the different multimers.
The semi-automated HYDRASYS 2 SCAN FOCUSING instrument performs all the steps needed to obtain gels ready for visual and densitometry-based interpretations.
Plasma samples
- Excellent reproducibility
- All multimer sizes
- Densitometry helps for results interpretation
- Patient’s profile overlayed with a normal control
Ready-to-use gels and reagents: 10 gels of 5 or 11 tests.
- HYDRAGEL 5 von WILLEBRAND MULTIMERS kit – P/N: 4359 to be associated to Revelation kit – P/N: 4747
- HYDRAGEL 11 von WILLEBRAND MULTIMERS kit – P/N: 4360 to be associated to Revelation kit – P/N: 4747 x2
Featured instruments for von Willebrand Multimer Analysis
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Related resources
See all resourcesvon Willebrand Disease Classification
There are three types of von Willebrand Disease. In this leaflet, different cases from congenital and acquired VWD are presented.
Apr. 11, 2022 Dec. 21, 2024
IFCC Euromedlab 2021
Sebia booth: #121
Sebia Workshop: EduW22
The contribution of multimer analysis in the diagnosis of von Willebrand disease
Educational Webinar with Dr. Annette BOWYER, Lead Biomedical Scientist
Jun. 27, 2022 Jun. 29, 2022
IFCC Worldlab 2022
Sebia booth: #72
Sebia Workshop: EduW25
Recommendations for the laboratory diagnosis of von Willebrand disease
Educational Webinar with Juliana A. Silva
Scientific publications
See all publicationsEvaluation of a semi-automated von Willebrand factor multimer assay
Algorithm for congenital and acquired von Willebrand disease includes Hydragel vWF multimer analysis.
Improving diagnosis of von Willebrand disease
Multimer analysis to improve von Willebrand disease classification with Hydragel vWF multimer Sebia.
von Willebrand Factor and COVID-19
Correlation of von Willebrand factor with the severity of COVID-9 progression.
Interested in von Willebrand Multimer Analysis?
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Carefully read the instructions in the reagent package inserts and instrument manuals.