Current Status of Detecting Monoclonal Antibodies and Free Light Chains in Serum
Educational Webinar with Dr. Matthias Orth
Abstract
Testing for abnormal immunoglobulins was based previously on serum electrophoresis and immunofixation only. In the last years, however, free light chain (FLC) testing in serum became one important pillar in the diagnosis and monitoring of Multiple Myeloma and related disorders such as Amyloidosis, most of these diseases occurring in older patients. In particular, the ratio of κ FLCs to λ FLCs became pivotal in the diagnosis, monitoring, and rule-out of patients with suspected myeloma. The challenge of this serum testing is the heterogeneity of free light chains and other methodical issues of testing which challenge in particular FLC tests based on turbidimetry and nephelometry.
The other challenges arise from the accumulation of FLC in serum due to the impaired excretion of FLC in patients with altered renal function – and renal function will decrease with age physiologically. Since elevated FLC will damage the kidneys, the establishment of reference ranges is challenging, and adequate reference ranges are necessary to obtain good discrimination between patients and normal (healthy) subjects. This webinar will describe the laboratory tests currently used and will suggest methods to establish reference ranges that allow the optimized use of FLC assays.
Speaker’s Biography
Matthias Orth received his training in Clinical Pathology at the universities of Freiburg and Magdeburg (Germany). From 1994-1997 he worked as a postdoctoral fellow at the Gladstone Institutes (GICD) of the University of San Francisco (UCSF). After his employment at the Free University Berlin and at Leipzig University, he became director of the Institute of Laboratory Medicine at Marienhospital Stuttgart in 2004. He is lecturer at the University of Heidelberg, Medical Faculty of Mannheim. He is active in the German Society of Laboratory Medicine as well as in IFCC, with a research focus on laboratory management. Very recently, he became chair of the Task Force Direct to Consumer testing of EFLM (TF-DTCT).
Speaker
More webinars
2005 Multiple Sclerosis Consensus Criteria
2005 Multiple sclerosis consensus criteria includes CSF IgG oligoclonal bands.
2017 Mc Donald Criteria for multiple sclerosis diagnosis
2017 Mc Donald Criteria for multiple sclerosis diagnosis includes CSF IgG oligoclonal bands.
AL Amyloidosis: Role of clinical lab in diagnosis and management
Educational Webinar with Prof. Giovanni PALLADINI, PhD
CAPILLARYS 3 DBS – Introductory video
The perfect match for screening hemoglobin disorders in newborns
Carbohydrate-deficient transferring testing: a truly traceable and specific biomarker for chronic alcohol misuse
Article from Jean Deenmamode, IFCC CDT working group chair in CLI magazine.
Catherine HERNANDEZ – Chemistry Production Manager
Find out what the role of a Chemistry Production Manager is.
Challenges in Implementing Capillary Electrophoresis for Immunotyping
Educational Webinar with Dr. Jack Maggiore from Loyola University Medical Center in Chicago, IL
Clinical and Operational Aspects of Measuring Hemoglobin A1c by Capillary Electrophoresis
Educational Webinar with David G. Grenache, PhD, D(ABCC)
Chief Scientific Officer, TriCore Reference Laboratories
Clinical use of FLC testing in Multiple Myeloma and AL Amyloidosis
Educational Workshop with Pr Giovanni Palladini
Complete your Myeloma diagnostic tools with Sebia FLC Assay
The serum Free Light Chains is an important test in Prognosis and Monitoring of Multiple Myeloma patients.
Current Status of Detecting Monoclonal Antibodies and Free Light Chains in Serum
Educational Webinar with Dr. Matthias Orth from University of Heidelberg at University Hospital Mannheim.
Discover how the automation track integration boosted the high potential of CAPILLARYS 3
With this short video report, become aware of all the benefits of partnering with Sebia and integrate your capillary electrophoresis into the lab track automation.
Discover the main applications related to the CDT marker
CDT is a sensitive and specific marker of chronic alcohol abuse, used in several contexts and notably including forensic settings, industries, clinical applications.
Early detection of Multiple Myeloma: The importance of serum protein electrophoresis
Educational Webinar with Dr. Julien GUILLEMAUD
Effects of hemoglobin C, D, E and S traits on measurements of hemoglobin A1c by twelve methods
Hemoglobin C, D Punjab, E or S trait can interfere with hemoglobin A1c (HbA1c) results depending on the method of analysis used. Therefore, it is important to know the limitations of the method used for HbA1c determination.
EurA1c trial
The EurA1c trial, organized yearly, investigates the performance of HbA1c assays across countries and manufacturers.
Evaluation of a semi-automated von Willebrand factor multimer assay
Algorithm for congenital and acquired von Willebrand disease includes Hydragel vWF multimer analysis.
Evaluation of Sebia’s isoelectrofocusing kit for A1AT
Evaluation of Sebia’s isoelectrofocusing kit for A1AT with HYDRASYS.
Experience and recommendations of a Major Cancer Center to use HYDRASHIFT daratumumab
Immunotherapies for Multiple Myeloma, like the monoclonal antibody daratumumab, can create false positive IgG Kappa bands on Immunofixation gels. This paper describes best practice guidelines for using the Hydrashift assay to remove daratumumab interference from Immunofixation gels.
From stand-alone to fully integrated Sebia solutions
Improve your lab performance by optimizing your Sebia solutions.
Further evaluation of the world health organization international reference reagent for Haemoglobin A2 measurement
This article assesses the performances of the different manufacturers and technologies (HPLC and CE) for Hb A2 measurement using both International Reference Reagent for HbA2 (WHO IRR 89/666) and analysis of three whole blood specimens over a range of HbA2, distributed to 56 laboratories located in 14 different countries.
Georges NOUADJE – R&D Manager
Find out what the role of a R&D Manager is.
Hemoglobinopathy Investigation – The Case for Capillary Electrophoresis
Educational Webinar with Jason EYRE
Hemoglobinopathy Testing: What are the Key Points a Lab Should Consider?
Educational Webinar Dr. Cornelia L. Harteveld from Leiden University Medical Center
HYDRASHIFT avoids monoclonal antibody interference on IMMUNOFIXATION
The HYDRASHIFT portfolio include IVD test to mitigate interferences seen in immunofixation result for patient treated with isatuximab or daratumumab.
May. 22, 2023 May. 24, 2023
IFCC Euromedlab /WorldLab 2023
Sebia booth: #21
Sebia Workshop: EduW15
Apr. 11, 2022 Dec. 21, 2024
IFCC Euromedlab 2021
Sebia booth: #121
Sebia Workshop: EduW22
Jun. 27, 2022 Jun. 29, 2022
IFCC Worldlab 2022
Sebia booth: #72
Sebia Workshop: EduW25
Impact of monoclonal antibody therapies on electrophoresis interpretation in myeloma patients
Educational Webinar with Dr. Rachel WHEELER
Improving diagnosis of von Willebrand disease
Multimer analysis to improve von Willebrand disease classification with Hydragel vWF multimer Sebia.
Improving the diagnosis of AATD with aid of Serum Protein Electrophoresis
Educational Webinar with Dr. Ilaria Ferrarotti
Jun. 10, 2022 Jun. 13, 2022
International Congress on Autoimmunity 2022
Booth: #23
International standardization of CDT measurement
IFCC CDT-Working Group standardized the CDT measurement.
Interview with M. Jean Deenmamode, CDT IFCC Working Group Chair
Interview between Sebia and M. Jean Deenmamode, CDT IFCC Working Group Chair
Modern Approach to Screening for Monoclonal Gammopathies Using Digital Workflows
Educational Webinar with Dr. Mark Zaydman from Washington University School of Medicine in St. Louis, MO
Multiple Myeloma clinical cases for diagnosis and monitoring according to latest IMWG recommendations
Educational Webinar with Prof. Alessandro Gozzetti, MD, PhD
Multiple Myeloma: « 20 » and « 100 » Ratios Cut-off applicable to Sebia FLC assays
The « 20 » and « 100 » Ratios Cut-off can be used in routine with Sebia FLC assays for high-risk smoldering multiple myeloma stratification and for assessing progression to active multiple myeloma or amyloidosis.
Newborn Screening for Sickle Cell Disease and other Haemoglobinopathies – MDPI – 2019
This special Issue related to newborn screening for Sickle Cell disease and other hemoglobinopathies tried to cover the most widely faced challenges in the field of newborn screening for SCD.
Nieves SANZ – Knowledge Center Advisor
Find out what the role of a Knowledge Center Advisor is.
Performance of HbA1c Assays in relation to clinical requirements for its use in diabetes
Educational Webinar with Dr. Cas WEYKAMP
Performance of Immunotyping vs. Immunofixation for the Detection of Monoclonal Gammopathies
Educational Webinar with Katie Thoren, PhD, Memorial Sloan Kettering Cancer Center
Pitfalls and ceveats of A1AT diagnosis
Pitfalls and caveats in α1-antitrypsin deficiency testing: a guide for clinicians.
Precision of CAPILLARYS 2 for the Detection of Hemoglobin Variants Based on Their Migration Positions
In this report, we evaluated the use of the capillary electrophoresis (CE) migration position in the CAPILLARYS 2 CE instrument.
Recommendations for the laboratory diagnosis of von Willebrand disease
Educational Webinar with Juliana A. Silva
Road-traffic accidents: CDT, a reliable marker for chronic alcohol abuse testing
Educational Webinar with Dr Nadia Maria Porpiglia
Sebia acquires Zeus Scientific
The strategic acquisition reinforces Sebia’s product portfolio & strategy in autoimmunity
Sebia and Metafora biosystems form strategic partnership to develop advanced in vitro diagnostics
Standardisation of the CDT measurement on CAPILLARYS 3 TERA
IFCC Standardisation of the Sebia CDT measurement on CAPILLARYS 3 TERA.
TAT reduction with solution integration
Understand your needs and constraints to provide you with the most suitable and integrated solution.
The contribution of multimer analysis in the diagnosis of von Willebrand disease
Educational Webinar with Dr. Annette BOWYER, Lead Biomedical Scientist
The impact of red blood cell lifespan on HbA1c measurement
This article reminds the reader of the importance of detecting the Hb variants when measuring HbA1c for the labs and clinicians to better interpret the results and to ensure optimal management of the diabetic patient.
The importance of CDT-IFCC, marker of chronic alcohol excessive consumption
Educational Webinar with M. Jean Deenmamode, IFCC Working Group Chair
The importance of SPE and A1AT phenotyping for a better AATD patient management
Educational Webinar Pr Simone Scarlata
The simple principles of innovative Capillary electrophoresis testing
Understand in 2 minutes how capillary electrophoresis works, and why it gives such high and clear resolution in lab testing.
von Willebrand Disease Classification
There are three types of von Willebrand Disease. In this leaflet, different cases from congenital and acquired VWD are presented.
von Willebrand Factor and COVID-19
Correlation of von Willebrand factor with the severity of COVID-9 progression.
Do you have questions about this webinar?
Contact us
This section contains information intended for wide distribution and may therefore contain product details or information that is not available or valid in your country.
Please contact your local Sebia representative. Information intended for healthcare professionals.
Carefully read the instructions in the reagent package inserts and instrument manuals.