Connective Tissues Diseases
Perfect discrimination between Connective Tissue Diseases.
About Connective Tissues Diseases
Autoimmune connective tissue diseases (CTD) are characterized by spontaneous overactivity of the immune system resulting in the production of antibodies against several autoantigens. The evolution of the disease can be rapid or slow and start from subtle abnormalities before ending in classical symptoms enabling the diagnosis. In order to understand by which CTD a patient is affected, the right combination of diagnostic tools and markers is necessary.
Antinuclear antibodies (ANA) and Extractable Nuclear Antigens (ENA) are valuable biomarkers for first line diagnostics, when inflammatory rheumatic diseases are suspected.
ANAs can be detected by indirect immunofluorescence assay (IFA) using HEp-2 cells or using an ELISA-based screen. Analysis of single ANA specificities is crucial for differential diagnosis of Systemic Lupus Erythematosus (SLE), Mixed Connective Tissue Disease (MCTD), Sjögren’s syndrome, Systemic Sclerosis (SSc), CREST syndrome, Polymyosits or Dermatomyositis and overlap syndrome. High titers of ANA, anti-dsDNA and anti-Sm are included in the International Criteria for SLE classification. For differentiation between different pathologies autoantibody specific immunoassays are used.
Key benefits
Fully automated ANA screening
Complete offer for ANA screening, including fully automated IFA solutions and ELISA-based screening (ANADetect/ANA screen).
Better and easier reading
IFA slides with clear vision of all phases of the cell cycle, regular cell distribution, excellent ratio of nucleus/cytoplasm.
Enhanced specificity
Special dsDNA preparation and innovative DNA binding technology for improved specificity.
Broad portfolio
One of the most comprehensive product portfolio enabling to differentiate CTD.
Full flexibility
Monotest technology for cost-efficient combination of the right differentiation assays.
Sebia's offer
ANA HEp-2 Test is an indirect immunofluorescence assay for the qualitative detection and semi-quantitative determination of IgG anti-nuclear antibodies in human serum by manual fluorescence microscopy or with the dIFine® automated microscope.
The presence of anti-nuclear antibodies can be used in conjunction with other serological tests and clinical findings to aid in the diagnosis of systemic lupus erythematosus SLE and other systemic rheumatic diseases CTD.
- IFA – FA2500A
dsDNA (Crithidia l.) is designed for the qualitative and semi-quantitative detection of antibodies to native DNA by the Indirect Fluorescent Antibody (IFA) technique and is for in vitro diagnostic use.
The anti-dsDNA antibodies have an important role in the development of Systemic Lupus Erythematosus (SLE) disease, and their affordable identification helps in the diagnostic path of this autoimmune condition.
- IFA – FA1001
ANA Detect is an immunoassay for the in vitro determination of IgG autoantibodies against 26 different antinuclear antigens (ANA) in human serum samples.
The assay is based on the following antigens: centromere B, histone proteins H1, H2A, H2B, H3, H4, histidyl-tRNA synthetase (Jo-1), mononucleosomes, Pm-Scl-100, polynucleosomes, RNP-A, RNP-C, RNP-70, RNP/Sm complex, DNA topoisomerase I (Scl-70 protein), SS-A 52 (Ro 52), SS-A 60 (Ro 60), SS-B (La), ssDNA , histone complex, Sm-BB, Sm-D, Sm-E, Sm-F, Sm-G and dsDNA.
The test is intended to aid in the diagnosis of CTD in conjunction with other laboratory and clinical findings.
- Alegria – ORG 200
- ELISA – ORG 600
ANAscreen is an immunoassay for the in vitro determination of IgG autoantibodies against 10 different antinuclear antigens (ANA) in human serum samples.
The assay is based on the following antigens: centromere B, histidyl-tRNA synthetase (Jo-1), RNP-70, RNP/Sm complex, DNA topoisomerase I (Scl-70 protein), Sm ribonucleoproteins, SS-A 52 (Ro 52), SS-A 60 (Ro 60) and SS-B (La).
The test is intended to aid in the diagnosis of CTD in conjunction with other laboratory and clinical findings.
- Alegria – ORG 238
- ELISA – ORG 538
Anti-alpha-Fodrin IgG is an immunoassay for the automated in vitro determination of IgG autoantibodies against alpha-fodrin in human serum samples.
The test is intended to aid in the diagnosis of CTD, especially Sjögren’s syndrome in conjunction with other laboratory and clinical findings.
- Alegria – ORG 242G
Anti-C1q is an immunoassay for the in vitro determination of IgG autoantibodies against complement protein C1q in human serum samples.
The test is intended to aid in the diagnosis of SLE and lupus nephritis in conjunction with other laboratory and clinical findings.
- Alegria – ORG 249
- ELISA – ORG 549
Anti-Centromere B is an immunoassay for the in vitro determination of IgG autoantibodies against centromere B in human serum samples.
The test is intended to aid in the diagnosis of CTD, including CREST syndrome in conjunction with other laboratory and clinical findings.
- Alegria – ORG 233
- ELISA – ORG 633
Anti-dsDNA IgG is an immunoassay for the in vitro determination of IgG autoantibodies against double-stranded DNA (dsDNA) in human serum samples.
The test is intended to aid in the diagnosis of CTD, especially SLE in conjunction with other laboratory and clinical findings.
- Alegria – ORG 204G
- ELISA – ORG 604
Anti-Histone is an immunoassay for the in vitro determination of IgG autoantibodies against histone proteins in human serum samples.
The test is intended to aid in the diagnosis of CTD, especially drug-induced lupus (DIL) in conjunction with other laboratory and clinical findings.
- Alegria – ORG 207
- ELISA – ORG 507
Anti-Jo-1 is an immunoassay for the in vitro determination of IgG autoantibodies against Jo-1 in human serum samples.
The test is intended to aid in the diagnosis of CTD, especially Polymyositis /Dermatomyositis (PM/DM) in conjunction with other laboratory and clinical findings.
- Alegria – ORG 213
- ELISA – ORG 513
Anti-Nucleosome is an immunoassay for the in vitro determination of IgG autoantibodies against nucleosomes in human serum samples.
The test is intended to aid in the diagnosis of SLE in conjunction with other laboratory and clinical findings.
- Alegria – ORG 228
- ELISA – ORG 528
Anti-Rib-P is an immunoassay for the automated in vitro determination of IgG autoantibodies against ribosomal P-proteins (Rib-P) in human serum samples.
The test is intended to aid in the diagnosis of SLE in conjunction with other laboratory and clinical findings.
- Alegria – ORG 217
Anti-RNP-70 is an immunoassay for the in vitro determination of IgG autoantibodies against RNP-70 in human serum samples.
The test is intended to aid in the diagnosis of CTD in conjunction with other laboratory and clinical findings.
- Alegria – ORG 232
- ELISA – ORG 632
Anti-RNP/Sm is an immunoassay for the in vitro determination of IgG autoantibodies against RNP/Sm in human serum samples.
The test is intended to aid in the diagnosis of systemic inflammatory autoimmune diseases CTD, especially SLE and MCTD in conjunction with other laboratory and clinical findings.
- Alegria – ORG 211
- ELISA – ORG 511
Anti-Scl-70 is an immunoassay for the in vitro determination of IgG autoantibodies against Scl-70 in human serum samples.
The test is intended to aid in the diagnosis of Systemic Scleroderma (SSc) in conjunction with other laboratory and clinical findings.
- Alegria – ORG 212
- ELISA – ORG 512
Anti-SS-A is an immunoassay for the in vitro determination of IgG autoantibodies against SS-A (52 and 60 kDa) in human serum samples.
The test is intended to aid in the diagnosis of CTD, especially Sjögren’s syndrome in conjunction with other laboratory and clinical findings.
- Alegria – ORG 208
- ELISA – ORG 508
Anti-SS-A 52 is an immunoassay for the in vitro determination of IgG autoantibodies against SS-A-52 in human serum samples.
The test is intended to aid in the diagnosis of CTD, especially Sjögren’s syndrome in conjunction with other laboratory and clinical findings.
- Alegria – ORG 252
- ELISA – ORG 652
Anti-SS-A 60 is an immunoassay for the in vitro determination of IgG autoantibodies against SS-A-60 in human serum samples.
The test is intended to aid in the diagnosis of CTD, especially Sjögren’s syndrome in conjunction with other laboratory and clinical findings.
- Alegria – ORG 260
- ELISA – ORG 660
Anti-SS-B is an immunoassay for the in vitro determination of IgG autoantibodies against SS-B in human serum samples.
The test is intended to aid in the diagnosis of CTD, especially Sjögren’s syndrome in conjunction with other laboratory and clinical findings.
- Alegria – ORG 209
- ELISA – ORG 509
Anti-ssDNA is an immunoassay for the in vitro determination of IgG autoantibodies against single-stranded DNA (ssDNA) in human serum samples.
The test is intended to aid in the diagnosis of CTD in conjunction with other laboratory and clinical findings.
- Alegria – ORG 205
- ELISA – ORG 605
ENA combi is an immunoassay for the in vitro determination of IgG autoantibodies against extractable nuclear antigens (ENA) in human serum samples.
The assay enables a distingused determination between antibodies against histidyl-tRNA synthetase (Jo-1), RNP/Sm complex, DNA topoisomerase I (Scl-70 protein), Sm ribonucleoproteins, SS-A 52 (Ro 52), SS-A 60 (Ro 60) and SS-B (La).
The test is intended to aid in the diagnosis of CTD in conjunction with other laboratory and clinical findings.
- ELISA – ORG 514
ENAscreen is an immunoassay for the in vitro determination of IgG autoantibodies against extractable nuclear antigens (ENA) in human serum samples.
The assay is based on the following antigens: histidyl-tRNA synthetase (Jo-1), RNP/Sm complex, DNA topoisomerase I (Scl-70 protein), Sm ribonucleoproteins, SS-A 52 (Ro 52), SS-A 60 (Ro 60) and SS-B (La).
The test is intended to aid in the diagnosis of CTD in conjunction with other laboratory and clinical findings.
- Alegria – ORG 206
- ELISA – ORG 506
Alegria – Check Connective Scr
- Alegria – ORG 160-2X05
Alegria-Check Connective Scr is a multi-parameter-specific serum control for daily internal quality control validated for the following assays:
- Anti-dsDNA IgG – ORG 204G
- ANAscreen – ORG 238
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Related resources
See all resourcesA quick and pocketable guide helping in the daily routine with HEp-2 cells
Sebia’s HEp-2 cells quality at a glance in a complete atlas of images.
HEp-2 cells: a reliable tool for antinuclear antibodies screening
A comprehensive overview of Sebia’s solutions for ANA screening for Connective tissue disease diagnosis.
Alegria 2 properties and workflow
Obtain your patients results with high flexibility reliability and efficiency with Alegria 2.
Customer Testimonial about the Alegria 2
The Alegria 2 provides the possibility of giving results to clinicians within a timeframe fitting clinical needs and helps to optimize the patient’s care pathway.
May. 17, 2024 May. 20, 2024
14th International Congress on Autoimmunity 2024
Sebia booth #15
Scientific publications
See all publicationsThe importance of testing anti-dsDNA, anti-nucleosome and anti-C1q antibodies in patients with systemic lupus erythematosus
The flexibility of Alegria monotest solution helps the correct stratification of the SLE patient population.
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