Hemoglobin disorders
screening
The high-resolution method for hemoglobin disorders screening.
About Hemoglobin disorders screening
Hemoglobin electrophoresis is a well-established technique routinely used in clinical laboratories to screen patient samples for hemoglobinopathies (hemoglobin variants and thalassemias).
Many international societies such as TIF(1), CDC and APHL(2) recognise the benefits of the Sebia Capillary Electrophoresis (CE) technology, such as the high resolution and its clear hemoglobin fractions profile in the screening of hemoglobin disorders.
In addition to the Capillary electrophoresis, Sebia provides Gel technique-based solutions: HYDRAGEL HEMOGLOBIN(E) and HYDRAGEL ACID(E) HEMOGLOBIN(E) that enable, on the HYDRASYS instruments the separation of the hemoglobins and the detection of the major hemoglobin variants: S or D and C or E, by electrophoresis on alkaline agarose gels.
Electrophoresis on acidic gels, like HYDRAGEL ACID(E) HEMOGLOBIN(E), should follow to confirm the identification of hemoglobin variants, mainly, to differentiate hemoglobins S from D and E from C.
The visualization of the gel provides qualitative and quantitative (HbA and HbA2) analysis.
Key benefits
Fully automated
CAPILLARYS instruments perform all sequences automatically, from primary tube sampling to final clear-cut profile, with precise quantification and presumptive identification of the most common hemoglobins.
High resolution
CAPILLARYS instruments allow high resolution separation of the major hemoglobin variants (Hb S, Hb C, Hb D and Hb E) and accurate quantification of the Hb A2 and Hb F.
Furthermore, the Sebia CE technique is highly reproducible and the migration position given by the software can be used as an aid in the identification of Hb variants(3).
Enhanced detection
Capillary zone electrophoresis allows clean separation of Hb E from Hb A2 and facilitates easier detection of Hb Bart’s and Hb H, the X-axis migration position is a great help in the presumptive identification of hemoglobin variants(3).
Adapted to each laboratory
Thanks to the broad range of capillary instruments, the hemoglobin technique answers the needs and variable workload of all laboratories involved in abnormal hemoglobin screening.
Main characteristics
- The HEMOGLOBIN(E) assay is based on the principle of capillary electrophoresis in free solution. Hemoglobin fractions are separated in silica capillaries, by their electrophoretic mobility and electroosmotic flow at a high voltage in an alkaline buffer.
- Hemoglobin fractions are directly detected at the specific absorbance of 415 nm. The pattern is divided into 15 zones (12 zones for cord blood). Each zone displays a drop-down library of possible variants migrating within this zone. More than 500 variants are listed.
Whole blood sample are used and are analysed directly on Capillary instruments.
Throughput (tests/hour)*
HEMOGLOBIN(E)
CAPILLARYS 3 OCTA 45
CAPILLARYS 3 TERA 65
CAPILLARYS 3 TERA MC3 176
MINICAP FLEX-PIERCING 7
* Using Software version 9.30
Each Sebia capillary instrument has a dedicated reagent kit to perform Hemoglobin fractions analysis.
- CAPI 3 HEMOGLOBIN(E) – P/N: 2507
- MINICAP HEMOGLOBIN(E) – P/N: 2207
Featured instruments for Hemoglobin disorders screening
See all instrumentsCAPILLARYS 3 OCTA
Evolution makes the difference.
CAPILLARYS 3 TERA MC
The power of up to 36 capillaries in one automated high volume workcell.
MINICAP FLEX-PIERCING
The compact and affordable automation of Capillary Electrophoresis.
Related resources
See all resourcesApr. 11, 2022 Dec. 03, 2024
IFCC Euromedlab 2021
Sebia booth: #121
Sebia Workshop: EduW22
Jun. 27, 2022 Jun. 29, 2022
IFCC Worldlab 2022
Sebia booth: #72
Sebia Workshop: EduW25
Hemoglobinopathy Testing: What are the Key Points a Lab Should Consider?
Educational Webinar Dr. Cornelia L. Harteveld from Leiden University Medical Center
Hemoglobinopathy Investigation – The Case for Capillary Electrophoresis
Educational Webinar with Jason EYRE
May. 22, 2023 May. 24, 2023
IFCC Euromedlab /WorldLab 2023
Sebia booth: #21
Sebia Workshop: EduW15
How External Quality Assessment supports quality improvement in laboratory hemoglobinopathy diagnosis
Educational Webinar with Dr. Barbara De la Salle
Scientific publications
See all publicationsPrecision of CAPILLARYS 2 for the Detection of Hemoglobin Variants Based on Their Migration Positions
In this report, we evaluated the use of the capillary electrophoresis (CE) migration position in the CAPILLARYS 2 CE instrument.
Further evaluation of the world health organization international reference reagent for Haemoglobin A2 measurement
This article assesses the performances of the different manufacturers and technologies (HPLC and CE) for Hb A2 measurement using both International Reference Reagent for HbA2 (WHO IRR 89/666) and analysis of three whole blood specimens over a range of HbA2, distributed to 56 laboratories located in 14 different countries.
Interested in Hemoglobin disorders screening?
(1) TIF (2016) – Prevention and Diagnosis of Haemoglobinopathies: A Short Guide for Health Professionals and Laboratory Scientists
(2) APHL (2015) – Hemoglobinopathies: Current Practices for Screening, Confirmation and Follow-up
This section contains information intended for wide distribution and may therefore contain product details or information that is not available or valid in your country.
Please contact your local Sebia representative. Information intended for healthcare professionals.
Carefully read the instructions in the reagent package inserts and instrument manuals.