Alpha 1-Antitrypsin (A1AT) Phenotype Testing
The gold standard assay for the alpha 1-antitrypsin (A1AT) phenotype testing.
About A1AT
The most common method to detect A1AT deficiency is protein phenotyping using isoelectric focusing.
The HYDRAGEL 18 A1AT ISOFOCUSING kit used on the HYDRASYS 2 SCAN FOCUSING instrument provides qualitative detection of the different phenotypes of the A1AT in human serum.
The semi-automated HYDRASYS 2 instrument enables migration, immunofixation and direct detection on agarose gels, to obtain A1AT profiles for qualitative analysis.
Phenotyping results in conjunction with clinical findings and other laboratory assays aid in the diagnosis of Alpha 1-antitrypsin deficiency.
Key benefits
Accurate Diagnosis
100% concordance with another agarose gel isocelectric focusing and in agreement with the different phenotypes.
Efficient Method
Clear band visualization for a reliable interpretation
Fast result
Simple step-by-step procedure without membrane transfer for 2.5 hours of turnaround time.
Testing is part of algorithms
HYDRAGEL 18 A1AT ISOFOCUSING is included in both screening and diagnostic algorithms to provide clinicians with phenotype of A1AT proteins secreted in blood circulation.
Main characteristics
The HYDRAGEL 18 A1AT ISOFOCUSING assay is based on the principle of agarose gel isoelectrofocusing followed by immunofixation.
- After the protein is separated according to their isoelectric point, the gel is directly incubated with a peroxidase labelled anti-A1AT antiserum with no need for an additional membrane blotting step.
- The gel is then processed to remove unbound proteins before the enzymatic reaction.
- The gel is stained and visually evaluated.
Serum can be used with HYDRAGEL 18 A1AT ISOFOCUSING.
- 2 stage-protocol: Isoelectrofocalisation and Immunofixation for an optimized result
- A1AT controls (MM, MZ and MS) are included in each series of analysis to aid in the indentification of M, S and Z phenotypes.
10 gels of 18 tests.
- HYDRAGEL 18 A1AT ISOFOCUSING – P/N: 4357
Featured instruments for A1AT
See all instrumentsHYDRASYS 2 SCAN FOCUSING
Large menu with high quality results on gel electrophoresis.
Related resources
See all resourcesScientific publications
See all publicationsPitfalls and ceveats of A1AT diagnosis
Pitfalls and caveats in α1-antitrypsin deficiency testing: a guide for clinicians.
Evaluation of Sebia’s isoelectrofocusing kit for A1AT
Evaluation of Sebia’s isoelectrofocusing kit for A1AT with HYDRASYS.
Interested in A1AT?
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Carefully read the instructions in the reagent package inserts and instrument manuals.