CNS Local IgG synthesis detection by isoelectrofocusing
The gold standard assay to detect CNS local IgG synthesis in multiple sclerosis.
About CNS Local IgG synthesis detection by IEF
Presence of intrathecal IgG suggests inflammatory disease of the Central Nervous System caused by multiple sclerosis (MS).
The HYDRAGEL CSF ISOFOCUSING kit used on the HYDRASYS 2 SCAN FOCUSING instrument provides qualitative detection and immunological characterization of IgG oligoclonal banding in human CSF (Cerebro Spinal Fluid) and serum. The semi-automated HYDRASYS instrument enables migration, immunofixation and direct detection on agarose gels, in order to obtain IgG profiles for qualitative analysis.
The main recommendations from the European Consensus Report for Multiple Sclerosis diagnosis (detailed in “Recommended Standard of Cerebrospinal Fluid Analysis in the Diagnosis of Multiple Sclerosis .A Consensus Statement.” Arch. Neurol.62,863-870,2005) are:
- Isoelectrofocusing (IEF) on agarose gel for immunoglobulins separation
- Use of non-concentrated CSF material
- Simultaneous analysis of the patient’s serum and CSF
- Identical IgG concentration in paired CSF-serum samples
The presence of CSF oligoclonal bands only, characterizing local IgG synthesis, tightly correlates with the diagnosis of Multiple Sclerosis. Their presence provides useful information when in agreement with the other clinical investigations.
Key benefits
Accurate Diagnosis of inflammatory disease of the CNS
Sensitivity increased about a 100-fold compared to standard immunofixation thanks to the detection with enzyme labelled antibodies.
Efficient Method
Clear oligoclonal band visualization for a reliable interpretation.
Faster result
Simple step-by-step procedure without membrane transfer for 3 hours of turnaround time.
Compliance with international consensus
HYDRAGEL CSF ISOFOCUSING meets all requirements needed to objectively detect CNS local IgG synthesis in multiple sclerosis.
Main characteristics
The HYDRAGEL CSF ISOFOCUSING assay is based on the principle of agarose gel isoelectrofocusing followed by immunofixation. The patient’s serum and CSF samples are analysed in parallel to compare the IgG distribution.
- Prior to performing the test, it is mandatory to quantify the IgG’s in both serum and CSF samples in order to adjust the IgG concentration to the same level.
- The proteins are first separated according to their isoelectric point, the gel is then incubated with a peroxidase labelled anti-IgG antiserum.
- The gel is then processed to remove unbound proteins prior to the enzymatic reaction.
- The gel is visually evaluated for the presence of CSF oligoclonal bands.
Serum and CSF samples are performed in parallel for each patient to compare IgG distribution as recommended by the European consensus.
- 2 stages-protocol: Isoelectrofocalisation and Immunofixation for an optimized result
- Enhanced software with high voltage monitoring
- Traceability and positive ID obtained by connecting the HYDRASYS instrument to the ASSIST pipetting station
Flexible Format of HYDRAGEL CSF ISOFOCUSING kit: 10 gels of 3 or 9 tests.
- HYDRAGEL 3 CSF ISOFOCUSING – P/N: 4353
- HYDRAGEL 9 CSF ISOFOCUSING – P/N: 4355
Featured instruments for CNS Local IgG synthesis detection by IEF
See all instrumentsHYDRASYS 2 SCAN FOCUSING
Large menu with high quality results on gel electrophoresis.
Related resources
See all resourcesScientific publications
See all publications2005 Multiple Sclerosis Consensus Criteria
2005 Multiple sclerosis consensus criteria includes CSF IgG oligoclonal bands.
2017 Mc Donald Criteria for multiple sclerosis diagnosis
2017 Mc Donald Criteria for multiple sclerosis diagnosis includes CSF IgG oligoclonal bands.
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