Serum and Urine
Immunofixation
Identification of monoclonal proteins by agarose gel electrophoresis.
About Serum and Urine Immunofixation
Immunofixation is an immunological method for detection and identification of monoclonal proteins in serum and urine. After the separation of serum and urine proteins according to their charge, the gel is incubated with different, specific antisera. The gel is then processed in order to remove the antisera excess prior to the final staining step. The gel is interpreted visually to type all immunoglobulins.
Key benefits
Clear separation
for protein fractions and identification of monoclonal component in serum and urine in agarose gel method.
Clear band visualisation
in serum and urine for quick identification of suspect monoclonal proteins IgG, IgA, IgM, Kappa (κ) or Lambda (λ) and free light chains involved in gammopathy.
High quality, ready to use antisera
for optimal reaction performance and accurate monoclonal component identification.
Semi-automated
HYDRASYS 2 SCAN FOCUSING system includes sample application, electrophoretic migration, immunofixation, staining, destaining and scanning connected to PHORESIS Software for data & result management.
Dedicate HYDRAGEL BENCE JONES kits
for detection of monoclonal components in urine samples.
Main characteristics
- The HYDRAGEL IF assay is based on the principle of agarose gel electrophoresis followed by immunofixation.
- After the separation of serum or urine proteins according to their charge, the gel is incubated with different specific, antisera for characterization of monoclonal component involved in the gammopathy.
Serum & Urine
Sensitivity
- The minimal detection limit for monoclonal immunoglobulins is about 120 mg/L for gels stained with acid violet and about 250 mg/L for gels stained with amidoblack.
- According to the mobility of the monoclonal component and polyclonal background, the sensitivity may vary.
Internal sensitivity study demonstrated that a Bence Jones protein can be detected in a serum at a concentration:
- as low as 20 mg/L with anti-free & bound light chains antisera
- as low as 50 mg/L with anti-free light chains antisera
- HYDRAGEL 1 IF (SM*) – P/N: 4801 / (DM**) – P/N: 4301
- HYDRAGEL 2 IF (SM) – P/N: 4802 / (DM) – P/N: 4302
- HYDRAGEL 4 IF (SM) – P/N: 4804 / (DM) – P/N: 4304
- HYDRAGEL 9 IF (SM) – P/N: 4809 / (DM) – P/N: 4309
- HYDRAGEL 1 BENCE JONES (SM) – P/N: 4821 / (DM) – P/N: 4321
- HYDRAGEL 2 BENCE JONES (SM) – P/N: 4822 / (DM) – P/N: 4322
- HYDRAGEL 4 BENCE JONES (SM) – P/N: 4824 / (DM) – P/N: 4324
* SM: Standard Mask
** DM: Dynamic Mask
Featured instruments for Serum and Urine Immunofixation
See all instrumentsHYDRASYS 2 SCAN FOCUSING
Large menu with high quality results on gel electrophoresis.
Related resources
See all resourcesPerformance of Immunotyping vs. Immunofixation for the Detection of Monoclonal Gammopathies
Educational Webinar with Katie Thoren, PhD, Memorial Sloan Kettering Cancer Center
Early detection of Multiple Myeloma: The importance of serum protein electrophoresis
Educational Webinar with Dr. Julien GUILLEMAUD
Moving from Immunofixation to Immunotyping Improves Standard of Care by Hand-on and Turnaround Time
Learn how a Sebia customer gained both clinically and operationally with a simplified IT workflow.
Challenges in Implementing Capillary Electrophoresis for Immunotyping
Educational Webinar with Dr. Jack Maggiore from Loyola University Medical Center in Chicago, IL
Scientific publications
See all publications
Interested in Serum and Urine Immunofixation?
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