Serum and Urine
Immunotyping
Fully automated monoclonal proteins identification by Capillarys electrophoresis.
About Serum and Urine Immunotyping
Immunotyping is used to detect and identify monoclonal proteins in serum and urine. Serum and urine proteins are mixed with specific antisera against Gamma- (IgG), Alpha- (IgA), Mu- (IgM) heavy chains Kappa (κ) and Lambda (λ) free and bound light chains.
Immunoglobulins specifically react with their corresponding antiserum to form a large Ab-AG complex that is removed and each antiserum pattern (IgG, IgA, IgM, κ and λ). A ELP (Protein Electrophoresis) reference curve is overlayed to show the disappearance of the abnormality in the antiserum-treated pattern, this removal indicates the presence of a monoclonal protein.
Key benefits
Fully automated testing
Qualitative analysis with fast separation and good resolution of serum and urine samples.
Direct visualization of serum and urine protein profile
for quick identification of suspected monoclonal proteins (IgG, IgA, IgM, Kappa (κ) and Lambda (λ)) involved in gammopathy.
High quality of antisera ready to use
for an optimum reaction performance and correct monoclonal component identification.
Scalable solutions
to best fit the need for all sized laboratories, from a standalone instrument to workcell configuration.
Main characteristics
- Based on the principles of capillary electrophoresis, Serum or Urine proteins are mixed with specific antisera.
- Immunoglobulins specifically react with their corresponding antiserum. At the end of the analysis, each antiserum pattern (IgG, IgA, IgM, κ and λ) is automatically overlaid with the ELP (Protein Electrophoresis) reference curve.
- Disappearance of the abnormality in the antiserum-treated pattern indicates the presence of a monoclonal protein.
Serum & Urine
- Full traceability and positive sample ID from primary tube samples
- Full traceability and reagents management through RFID tags for both reagents and antisera
- On board temperature controlled storage for antisera vials
- Full automation
- Easy identification of reagent status via color-coded, backlit reagent containers
Sensitivity
- Monoclonal proteins can be detected at a concentration as low as 12.5 mg/dL in serum. According to the mobility of the monoclonal component and polyclonal background, the sensitivity may vary.
- Protein abnormality limit of detection is approximately 9 mg/L with CAPI 3 URINE.
Throughput (tests/hour)*
IMMUNOTYPING
CAPILLARYS 3 OCTA (serum or urine) 9
CAPILLARYS 3 TERA (serum or urine) 11
CAPILLARYS 3 TERA MC3 (serum or urine) 27
MINICAP FLEX-PIERCING (serum) 1.7
* Using Software version 9.30
- CAPI 3 IMMUNOTYPING – P/N: 2600
- MINICAP IMMUNOTYPING – P/N: 2300
Featured instruments for Serum and Urine Immunotyping
See all instrumentsCAPILLARYS 3 OCTA
Evolution makes the difference.
CAPILLARYS 3 TERA MC
The power of up to 36 capillaries in one automated high volume workcell.
MINICAP FLEX-PIERCING
The compact and affordable automation of Capillary Electrophoresis.
Related resources
See all resourcesPerformance of Immunotyping vs. Immunofixation for the Detection of Monoclonal Gammopathies
Educational Webinar with Katie Thoren, PhD, Memorial Sloan Kettering Cancer Center
Early detection of Multiple Myeloma: The importance of serum protein electrophoresis
Educational Webinar with Dr. Julien GUILLEMAUD
Moving from Immunofixation to Immunotyping Improves Standard of Care by Hand-on and Turnaround Time
Learn how a Sebia customer gained both clinically and operationally with a simplified IT workflow.
Challenges in Implementing Capillary Electrophoresis for Immunotyping
Educational Webinar with Dr. Jack Maggiore from Loyola University Medical Center in Chicago, IL
Scientific publications
See all publications
Interested in Serum and Urine Immunotyping?
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Carefully read the instructions in the reagent package inserts and instrument manuals.