About
The ELISA reaction relies on the specific interaction between antibodies and antigens. Wells in ELISA plates are coated with test-specific antigens. After a wash cycle removes excess and non-specifically bound antibodies, a secondary antibody with an enzyme detects the generated immune complex. Another wash cycle removes excess of the enzyme conjugate before a colorless substrate is added and converted into a colored product by the enzyme. The color intensity is measured photometrically. It is proportional to the antibody activity in the sample. A standardized master curve is used for evaluation. Antibody activities of unknown samples are derived from their optical signals via the recalibrated standard curve.
Sebia’s Expertise
For over 35 years, ORGENTEC Diagnostika GmbH and Zeus Scientific have been developing, producing and distributing ELISA test systems for autoimmune- and infection diagnostics. The experience of these companies is now complementing the Sebia ELISA portfolio. This product line already includes more than 50 classical ELISA kits and more than 100 corresponding test systems for automated processing with Alegria.
Differential diagnosis in autoimmunity often includes testing for particular infectious diseases. For this purpose, Sebia offers Alegria test kits for infectious disease diagnostics.
Featured resources on ELISA
See all resourcesAnti-MCV – Fill the gap in rheumatoid arthritis diagnosis
This comprehensive meta-analysis highlights the benefit of testing anti-MCV additionally to anti-CCP and RF in the context of early diagnosis of Rheumatoid Arthritis.
The importance of testing anti-dsDNA, anti-nucleosome and anti-C1q antibodies in patients with systemic lupus erythematosus
The flexibility of Alegria monotest solution helps the correct stratification of the SLE patient population.
Automated analysis of stool biomarkers with Alegria
Alegria fecal calprotectin reduces TAT helping in the diagnosis of inflammatory bowel diseases with robust results.
Automated analysis of a comprehensive profile of stool and serological biomarkers with Alegria
A fully dedicated test portfolio to the diagnosis of inflammatory bowel diseases.
Upcoming events
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Rheumatoid Arthritis
A holistic portfolio with Anti-MCV to unlock the full potential of early diagnosis.
Connective Tissues Diseases
Perfect discrimination between Connective Tissue Diseases.
Antiphospholipid Syndrome
Unique accuracy in Antiphospholipid Syndrome Diagnostics.
Celiac disease
Unmasking celiac disease, empowering health.
Thyroid Diseases
Accuracy in thyroid autoimmune disease diagnostics.
Autoimmune Liver and Gastric Diseases
A complete solution for the diagnosis of gastric and liver autoimmune diseases.
Vitamin D
Accurate and reliable Vitamin D level measurements.
Infectious Diseases
Comprehensive Infectious and Autoimmune Diseases diagnostics on one device.
Stool Assays
Stool assays: Detect inflammation, protect patients’ gut!
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Please contact your local Sebia representative. Information intended for healthcare professionals.
Carefully read the instructions in the reagent package inserts and instrument manuals.